Other

Part:BBa_J100255:Design

Designed by: Helen Webster   Group: Campbell M Lab   (2016-04-12)


tCloneTet+PDB riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 351
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 497
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 523
    Illegal NgoMIV site found at 891
    Illegal NgoMIV site found at 1051
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

J00207 is the RS3 riboswitch into which the PDB aptamer sequence was inserted. tCloneTet came from the J119374 plasmid.

References